An Aqueous Extract of Rosmarinus officinalis Induced Hepatotoxicity in Male Albino Rats
Abstract
Background: Because it induces oxidative stress and the loss of cellular glutathione, acetaminophen (paracetamol) is still a leading cause of drug-induced liver injury (DILI) worldwide. Further investigations are required to prove the unique hepatoprotective effect of the aqueous extract of the medicinal plant Rosmarinus officinalis (Rosemary) on Paracetamol toxicity. Rosemary is well-known for its antioxidant properties. Objective: To find out if an aqueous extract of Rosmarinus officinalis can protect male albino rats from hepatotoxicity. Methods: One hundred twenty-four rats aged 13-14 weeks and weighing from 225-250 grams were used for the research. There were 6 rats per each of 4 groups. G1, the control group that did not receive any treatment, was the first one. The second group G2 was the control group who were given 25 mg/kg of paracetamol. The third group (G3) was treated with aqueous rosemary extract (200 mg/kg). The oral dose of Paracetamol (25 mg/kg) was given to each group every 2 hours after giving an aqueous extract (200 mg/kg) in Group 4 (control group). All oral administration was made through the gavage tube in the 60 day trial. Results: When paracetamol was dosed, there was a significant reduction (P ≤ 0.05) in average GSH and CAT levels and significant rise (P ≤ 0.05) in average MDA, ALT, and ALP levels as well as significant increase in the liver impairment score, indicating that this group was better than the control group. In the control group, no significant difference was found between the levels of AST, ALT and ALP at 200 mg/kg ROAE. With 200 mg/kg ROAE+25 mg Paracetamol group, however, there was a significant decrease in AST, ALT and ALP when compared with Paracetamol group. This indicates antioxidant and enzyme activity has been restored to normal level after pre-treatment with the aqueous rosemary extract. The significant increase in GSH and CAT levels and the significant decrease in MDA levels in the ROAE + paracetamol group when compared with the control group indicated that there was a remarkable improvement in the activity of these antioxidant enzymes. Histological examination showed that significant cell infiltration and central tubule degradation were present in the group receiving paracetamol. On the other hand, the groups that were given rosemary showed significant improvement. Preserved hepatocyte morphology, decreased necrotic areas and minimal inflammatory response indicated a strong regenerative response. Conclusion: Based on its bioactive component content, which helped stabilize metabolism and neutralize hepatocyte membrane, the study found that the aqueous rosemary extract had a hepatoprotective effect against paracetamol injury.
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References
Bergmeyer, H. U.; Herder, M.; & Ref, R. (1986). International federation of clinical chemistry (IFCC). J. clin. Chem. clin. Biochem, 24(7), 497-510.
Bomgning, C. L. K., Sinda, P. V. K., Ponou, B. K., Fotio, A. L., Tsague, M. K., Tsafack, B. T., ... & Nguelefack, T. B. (2021). Hepatoprotective effects of extracts, fractions and compounds from the stem bark of Pentaclethra macrophylla Benth: Evidence from in vitro and in vivo studies. Biomedicine & Pharmacotherapy, 136, 111242.
Bosch, M. E., Sánchez, A. R., Rojas, F. S., & Ojeda, C. B. (2006). Determination of paracetamol: Historical evolution. Journal of pharmaceutical and biomedical analysis, 42(3), 291-321.
Burtis, C. A., and Ashood, E. R. (1999). Text book of clinical chemistry 3 rd ed. Vol.(2) W. Sanders Company, 1003, 1059–1060.
Elmetwally, E. M., Mousa, Z. M., Ibrahim, S. R., & Gohari, S. T. (2024). Protective effect of Gymnema sylvestre and Rosmarinus officinalis leaves against hepatorenal toxicity of Paracetamol in experimental rats. , 10(2), 407-431.
Fadlalla, E. A. S., & Galal, S. M. (2020). Hepatoprotective and reno-protective effects of artichoke leaf extract and rosemary extract against Paracetamol induced toxicity in Albino Rats. Journal of Pharmaceutical Research International, 32(32), 67-81.
Guimarães, N. S., Ramos, V. S., Prado-Souza, L. F., Lopes, R. M., Arini, G. S., Feitosa, L. G., ... & Rodrigues, T. (2023). Rosemary (Rosmarinus officinalis L.) glycolic extract protects liver mitochondria from oxidative damage and prevents acetaminophen-induced hepatotoxicity. Antioxidants, 12(3), 628.
Hadwan, M. H., and kadhum Ali, S. (2018). New spectrophotometric assay for assessments of catalase activity in biological samples. Analytical Biochemistry, 542, 29–33.
Hajam, Y. A., Rani, R., Ganie, S. Y., Sheikh, T. A., Javaid, D., Qadri, S. S., ... and Reshi, M. S. (2022). Oxidative stress in human pathology and aging: molecular mechanisms and perspectives. Cells, 11(3), 552.
Hasanein, P., & Sharifi, M. (2017). Effects of rosmarinic acid on acetaminophen-induced hepatotoxicity in male Wistar rats. Pharmaceutical biology, 55(1), 1809-1816.
Hernández-Pérez, M., R. E. López-García, R. M. Rabanal, V. Darias, and A. Arias. (1994). “Antimicrobial Activity of Visnea Mocanera Leaf Extracts.” Journal of Ethnopharmacology 41(1–2):115–19
Iloamaeke, I. M., and Iwuozor, O. K. (2018). Quality assessment of selected paracetamol tablets sold at bridge head market, Onitsha, Nigeria. Br J Pharm Med Res, 3(5), 8.
Kaman, P. K., and Dutta, P. (2019). Synthesis, characterization and antifungal activity of biosynthesized silver nanoparticle. Indian Phytopathology, 72, 79–88.
Kanakamani, S., Uthamaramasamy, S., & Mangalanathan, M. (2018). In vitro screening of anti-inflammatory potential of Mirabilis jalapa Linn flowers and Abelmoschus esculentus leaves. Int J Curr Res, 10(3), 67257-60.
Kola, A., Vigni, G., Lamponi, S., & Valensin, D. (2024). Protective contribution of rosmarinic acid in rosemary extract against copper-induced oxidative stress. Antioxidants, 13(11), 1419.
Kowsalya, R., Kaliaperumal, J., Vaishnavi, M., & Namasivayam, E. (2015). Anticancer activity of Cynodon dactylon L. root extract against diethyl nitrosamine induced hepatic carcinoma. South Asian journal of cancer, 4(02), 083-087.
Malo, C., Gil, L., Cano, R., Martínez, F., and Galé, I. (2011) Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology, 75(9),1735-1741.
Mecarthy, T; Kerry,J; Kerry, J; Blynch, P; Buckley, D .(2001) .Evaluation of antioxidant potential of natural food / plant extracts compared with synthetic antioxidant and vitamin E, in raw and cooked pork patties. Meat Sci. 57, 45-52.
Moder, K. (2010). Alternatives to F-test in one way ANOVA in case of heterogeneity of variances (a simulation study). Psychological Test and Assessment Modeling, 52(4), 343.
Muslih, B.; Mizil, Y. O.; and Al-Nimmer, M. S. (2001). Detection the level of peroxynitrite and related with antioxidant status in the serum of patiens with acute myocardial infraction. National.J.Chemistry, 4, 625–637.
Ogemdi, I. K. (2019). A Review on the Properties and Uses of Paracetamol. International Journal of Pharmacy and Chemistry, 5(3), 31-35
Rajesh, M. G., & Latha, M. S. (2004). Preliminary evaluation of the antihepatotoxic activity of Kamilari, a polyherbal formulation. Journal of Ethnopharmacology, 91(1), 99-104.
Rašković, A., Milanović, I., Pavlović, N., Ćebović, T., Vukmirović, S., & Mikov, M. (2014). Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotective potential. BMC complementary and alternative medicine, 14(1), 225.
Reagan‐Shaw, S., Nihal, M., and Ahmad, N. (2008). Dose translation from animal to human studies revisited. The FASEB journal, 22(3), 659-661
Saleem, T. M., Chetty, C. M., Ramkanth, S. V. S. T., Rajan, V. S. T., Kumar, K. M., & Gauthaman, K. (2010). Hepatoprotective herbs—a review. Int J Res Pharm Sci, 1(1), 1-5.
Shao, H. B., Chu, L. Y., Lu, Z. H., & Kang, C. M. (2007). Primary antioxidant free radical scavenging and redox signaling pathways in higher plant cells. International journal of biological sciences, 4(1), 8.
Suvarna, S. K.; Layton, C.; & Bancroft, J. D. (2013). Bancroft’s Theory and Practice of Histological Techniques. In Elsevier, 11–36.
Wahid, A., Hamed, A. N., Eltahir, H. M., & Abouzied, M. M. (2016). Hepatoprotective activity of ethanolic extract of Salix subserrata against CCl4-induced chronic hepatotoxicity in rats. BMC complementary and alternative medicine, 16(1), 263.
Wang, X., Wu, Q., Liu, A., Anadón, A., Rodríguez, J.-L. Martínez-Larrañaga, M.-R., Yuan, Z., and Martínez, M.-Á. (2017). Paracetamol: Overdose-induced oxidative stress toxicity, metabolism, and protective effects of various compounds in vivo and in vitro. Drug Metabolism Reviews,49(4), 395-437.
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