Bacteriocin Production by Escherichia coli Isolated from Clinical Samples

Haneen Musa Jawad (1)
(1) Al Qasim Green University, College of Biotechnology, Department of Medical Biotechnology. , Iraq

Abstract

The issue of antibiotic resistance has been becoming worse recently, therefore it's critical to discover new treatments for diseases brought on by microorganisms that current medications are unable to eliminate. Bacteriocin, a class of small antimicrobial peptides (AMPs), has demonstrated efficacy against a wide range of multidrug-resistant microorganisms, suggesting that it may be a viable antibiotic substitute. Apart from its minimal cytotoxicity against human cells and strong selectivity against microbial infections, the majority of bacteriocins exhibit stability and resilience to pH and temperature changes as well as enzyme breakdown. Small peptides known as bacteriocins have a wide range of structures and purposes, but little is known about how they work. The bacteriocin that is created by E. coli will be discussed in this study, along with its modes of action, the culture conditions that are employed to enhance its production, and the cutting-edge methods that are utilized to discover them. The use of bacteriocin as novel cancer treatment agents and food preservatives will also be covered.

Full text article

Generated from XML file

References

Abdul-hussein, Z. K., et al. (2018). "Molecular Diagnosis of Diarrheagenic E. coli Infections Among the Pediatric Patients in Wasit Province, Iraq." Journal of Pure & Applied Microbiology 12(4).

Al-Dulami, H. (1999). Effect of crude colicin extracted from Escherichia coli on immune cells, M. Sc Thesis, Collage of Science. Al–Mustensiriya University.

Al-Saeedi, M., et al. (2019). "Standardized endocystectomy technique for surgical treatment of uncomplicated hepatic cystic echinococcosis." PLoS neglected tropical diseases 13(6): e0007516.

Albadri, W. M., et al. (2021). "The relationship between the shear strength and water retention curve of unsaturated sand at different hydraulic phases." Acta Geotechnica 16: 2821-2835.

Alqaisi, A. H. (2023). "SOME CHARICTERISTIC OF MICROBIAL INDOLE EXTRACTED FROM PATHOGENIC E. COLI IN COMPARABLE WITH STANDARD ONE." Iraqi Journal of Agricultural Sciences 54(5): 1193-1201.

Bradley, D. E. (1967). "Ultrastructure of bacteriophage and bacteriocins." Bacteriological reviews 31(4): 230.

Brink, B. (2010). "Urease test protocol." American Society for Microbiology: 1-7.

Bures, J., et al. (1986). "Colicinogeny in colorectal cancer." Neoplasma 33(2): 233-237.

Cabo, M., et al. (1999). "A method for bacteriocin quantification." Journal of Applied Microbiology 87(6): 907-914.

Cascales, E., et al. (2007). "Colicin biology." Microbiol Mol Biol Rev 71(1): 158-229.

Cavard, D., et al. (1987). "Lipoprotein nature of the colicin A lysis protein: effect of amino acid substitutions at the site of modification and processing." Journal of bacteriology 169(5): 2187-2194.

Charkhian, H., et al. (2024). "Assessment of bacteriocin production by clinical Pseudomonas aeruginosa isolates and their potential as therapeutic agents." Microbial Cell Factories 23(1): 175.

Cursino, L., et al. (2002). "Recent updated aspects of colicins of Enterobacteriaceae." brazilian journal of microbiology 33: 185-195.

Dash, P., et al. (2016). "Practical Manual: Winter School on Marker free transgenics and methods of transgene detection sponsored by ICAR; 1-21 February, 2016." ICAR-National Research Centre on Plant Biotechnology, New Delhi, India 125.

Gomez, A. O., et al. (2023). "A Wild Pseudomonas has appeared: An Exercise in Bacterial Isolation and Identification." International Journal of Undergraduate Research and Creative Activities 15(1): 11.

Harry, C. O. and D. Walker (2013). "Cytotoxic activity of colicin E1, E3 and E9 against E. coli BW25113 in the planktonic and biofilm states." Int. J. Curr. Res. Aca. Rev 1(2): 55-71.

Hemraj, V., et al. (2013). "A review on commonly used biochemical test for bacteria." Innovare Journal of Life Science 1(1): 1-7.

Hijazi, M. M., et al. (2024). "The importance of the bacterial spectrum in the clinical diagnostics and management of patients with spontaneous pyogenic spondylodiscitis and isolated spinal epidural empyema: a 20-year cohort study at a single spine center." BMC Infectious Diseases 24(1): 39.

Jerman, B., et al. (2005). "Sublethal concentrations of ciprofloxacin induce bacteriocin synthesis in Escherichia coli." Antimicrob Agents Chemother 49(7): 3087-3090.

Kalyan, R. (2018). "COMMON BIOCHEMICAL TESTS USED IN MICROBIOLOGY." Microbiology Practical Manual, -E-book: 35.

Kang, J. and M. Lee (2005). "Characterization of a bacteriocin produced by Enterococcus faecium GM‐1 isolated from an infant." Journal of Applied Microbiology 98(5): 1169-1176.

Kapoor, G., et al. (2017). "Action and resistance mechanisms of antibiotics: A guide for clinicians." Journal of Anaesthesiology Clinical Pharmacology 33(3): 300-305.

Klangsin, P. (2000). Removal of fecal coliform bacteria by zebra mussels, University of Michigan.

Kohlerschmidt, D. J., et al. (2021). Identification of aerobic Gram-negative bacteria. Practical handbook of microbiology, CRC Press: 59-70.

Laird, E. D. (2016). Characterization of antibiotic resistance profiles of surface water bacteria in an urbanizing watershed.

Loy, A., et al. (2002). "Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment." Applied and Environmental Microbiology 68(10): 5064-5081

MacFaddin, J. (2000). "Biochemical tests for identification of medical bacteria, williams and wilkins." Philadelphia, PA 113(7).

MacWilliams, M. P. (2009). "Citrate test protocol." American Society for Microbiology: 1-7.

Mader, A., et al. (2015). "Amount of colicin release in Escherichia coli is regulated by lysis gene expression of the colicin E2 operon." PloS one 10(3): e0119124.

Miller, H. A. (2024). "Modifying the Gram Stain Lab for Skill Proficiency and Conceptual Understanding." The American Biology Teacher 86(3): 161-166.

Nikunjkumar, B. D. (2012). "Molecular identification of bacteria using 16s rDNA sequencing." Gujarat University, Gujarat, India.

Orekan, J., et al. (2021). "Culture media for clinical bacteriology in low-and middle-income countries: challenges, best practices for preparation and recommendations for improved access." Clinical Microbiology and Infection 27(10): 1400-1408.

Pugsley, A. P. (1983). "Autoinduced synthesis of colicin E2." Molecular and General Genetics MGG 190(3): 379-383.

Pugsley, A. P. and M. Schwartz (1983). "A genetic approach to the study of mitomycin-induced lysis of Escherichia coli K-12 strains which produce colicin E2." Molecular and General Genetics MGG 190(3): 366-372.

Pugsley, A. P. and M. Schwartz (1984). "Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase." The EMBO journal 3(10): 2393-2397.

Ridley, H. and J. H. Lakey (2015). "Antibacterial toxin colicin N and phage protein G3p compete with TolB for a binding site on TolA." Microbiology 161(Pt_3): 503-515.

Riley, M. A. (2011). Bacteriocin-mediated competitive interactions of bacterial populations and communities. Prokaryotic antimicrobial peptides: from genes to applications, Springer: 13-26.

Shahu, S., et al. (2024). "Bridging DNA contacts allow Dps from E. coli to condense DNA." Nucleic Acids Research 52(8): 4456-4465.

Srinivasan, R., et al. (2015). "Use of 16S rRNA gene for identification of a broad range of clinically relevant bacterial pathogens." PloS one 10(2): e0117617.

Tarelli, G. T., et al. (1994). "Production of bacteriocins active against Listeria monocytogenes and Listeria innocua from dairy enterococci." Food Microbiology 11(3): 243-252.

Tarrand, J. J. and D. Gröschel (1982). "Rapid, modified oxidase test for oxidase-variable bacterial isolates." Journal of clinical microbiology 16(4): 772-774.

Terlizzi, M. E., et al. (2017). "UroPathogenic Escherichia coli (UPEC) infections: virulence factors, bladder responses, antibiotic, and non-antibiotic antimicrobial strategies." Frontiers in microbiology 8: 1566.

Thonda, O. A., et al. (2024). "Microbiological and Randomly Amplified Polymorphic DNA (RAPD) Marker Protocol for Silicone Condom Lubricant Isolates." Science and Technology Development Journal 27(Online First): press-press.

Tille, P. (2015). Bailey & Scott's diagnostic microbiology-E-Book, Elsevier Health Sciences.

Wang, X. and Q. Song (2024). "Clinical Situation, Species Distribution, and Antibiotic Resistance of Pathogenic Bacteria and Fungi in 626 Cases of Vulvovaginitis in Hangzhou, China." Clinical Laboratory 70(5).

Wanger, A., et al. (2017). "Microbiology and molecular diagnosis in pathology: a comprehensive review for board preparation, certification and clinical practice."

Wilfinger, W. W., et al. (2006). "Assessing the quantity, purity and integrity of RNA and DNA following nucleic acid purification." DNA sequencing II optimizing preparation and cleanup 291: 312.

Authors

Haneen Musa Jawad
Jawad, H. M. (2024). Bacteriocin Production by Escherichia coli Isolated from Clinical Samples. Journal of Current Medical Research and Opinion, 7(08), 3401–3416. https://doi.org/10.52845/CMRO/2024/7-8-3

Article Details