Biodegradation of Paper Waste by Fusarium spp.

Abstract

This study was conducted to detect and identify most important of Fusarium isolates that can degrades waste paper in environment, detection their ability to production Cellulase enzyme who, is responsible in biodegradation process. Fielded inspection and isolation and identification process revealed three species Fusarium oxysporum, F.  solani, and F. pllyphialidicum were the most important in waste biodegradation, while F. roseum was the less in biodegradation ability. Grown tested on solid waste paper showed Fusarium oxysporum was the most capable of growing in mediumin ratio near by 78 % than followed by F.  solani in 63 % and  F. pllyphialidicum was 50 % comperin with F. roseum  which was the less one in capability in growing on solid waste paper medium.

The growth results of the isolates on liquid media were confermed with the results of solid media, as the fungus F. oxysporum ranked first in growth based on the weight of the bio mass with significant differences which was 255.1 mg compering with the latest isolate F. roseum  119.6 mg, while F.  solani was the second one with significant differences reached to223.4 mg with last one, also F. pllyphialidicum has a 189.2 gm significant differences with F. roseum. Chromogenic method by using Gongo red stain was apply to detect efficiency of Fusarium species to producing cellulose enzyme to degradation waste paper considering clear zone as indicator to cellulose analysis, F. oxysporum has a significant impact with length of clear zone was 31 mm compering with the latest isolate F. roseum 19 mm, then F.  solani was the second one with significant differences was 26 mm and the third was F. pllyphialidicum compering with F. roseum 19 mm.