Ruta graveolens in vitro Anti-fungal Activity and Screening of Bioactive Chemical Compounds Using Fourier transform infrared spectroscopic

Abstract

The objectives of this study were analysis of the secondary metabolite products and evaluation of Anti-fungal activity. The FTIR analysis of Ruta graveolens proved the presence of functional group assignment Alkyl halides, Alkenes, Aromatic, and Amide, with Intensity 56.899 (Strong), 73.673 (Strong), 71.567 (Strong), 52.162 (Strong), 51.019 (Strong), 77.325 (Strong), 77.311 (Strong), 73.531 (Medium), 72.276 (Bending), 83.341 (Unknown), 77.583 (Unknown), 73.851 (Bending), 72.556 (Bending).  and Peak (Wave number cm-ˡ) 667.37, 873.75, 921.97, 1004.91, 1014.56, 1242.16, 1317.38, 1595.13, 1614.42, 2330.01, 2357.01, 3228.84 and 3282.84. anti-fungal activity of R. graveolens methanolic extract was evaluated by determining the zone of inhibition against fungi. R. graveolens was very highly active against A. fumigatus  (3.71±0.19).