Detection and Characterization of Virulence and Resistance Determinants (spa, lukS/F-PV, mecA) in Staphyllococus aureus from Mastitic Cow Milk

Abstract

Antibiotic resistance is one of the huge medical problems causes by which mastitis cuases the most economically important disease affecting dairy cattle worldwide. The current study aimed to molecular detection and characterization of selected virulence and antimicrobial resistance genes (spa, many lukS/F-PV, mecA) in Staphylococcus aureus isolates obtained from cow mastitis milk in Diyala Governorate. Hundred mastitis milk samples were collected from cows affected with clinical mastitis. Molecular positivity rate was 30.0%, total 30 positive isolates were identified from the tested 100 mastitis milk samples. Conventional PCR analysis showed that 30 (100 %), 19 (63.3 %) and 7 (23.3 %) isolates were positive for spa, mecA and lukS/F-PV respectively. The results obtained confirmed the circulation of virulence- and methicillin-resistance-genes among strains of Staphylococcus aureus isolated from bovine mastitis. Sequencing and sequence alignment of selected PCR positive amplicons at Macrogen Company showed 99% or more similarity with GenBank reference strains.  By this study we can conclude that the impact  of both molecular characterization and epidemiological surveillance for bovine mastitis-associated S. aureus isolates virulence gene and antibacterial susceptibility test  through PCR and gene sequencing.