Effect of Continuous Laser on The Number Lymphocyte Cells and its DNA
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Abstract
The aim of this research is to evaluate the effects of a helium-neon laser on lymphocyte cells and DNA. Many different medical applications make extensive use of laser light. Like other lasers, the He-Ne laser's applicability in medicine is dependent on how laser light interacts with the biological system. The objective is to demonstrate the impact of 632.8 nm helium-neon (He-Ne) laser irradiation on human lymphocyte blood cells and their DNA. Techniques Eighty blood samples from individuals who seemed to be in good health were used in this investigation. The samples were split into two groups: thirty samples from the first group were processed solely for the purpose of separating lymphocyte blood cells, and fifty samples from the second group were used to assess the impact of He-Ne laser irradiation on the DNA extracted from the lymphocyte blood cells. Outcomes There was a significant difference (P < 0.05) in the survival percentage of lymphocyte cells (99.0%, 98.84%, 99.78%, and 98.78%) at the employed doses of He-Ne laser (19, 36, 53.5, and 70 J/m²) compared to those cells that were not exposed to He-Ne laser irradiation. The following doses (19, 36, and 70 J/m²) were applied to the extracted DNA immediately following the He-Ne laser irradiation alone. The DNA showed significant damage, with the fraction of DNA survival percentages being (85.5%, 88.5%, and 87.1%), respectively, and a significant difference (P < 0.05) between the DNA survival before and after the He-Ne laser irradiation. In summary with longer exposure times (3. 5, 7, and 9 s) and higher He-Ne laser doses, the fraction of lymphocytes that survive is declining. Independent of the irradiation dosages, He-Ne laser irradiation damages DNA to a considerable extent.
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