Anti-Microbial activity and Chemical constituents of Ethanolic Leaves Extract of Ocimum basilicum L., Lamiaceae Using GC-MS Technique
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Anti-Microbial activity, Chemical constituents, Ocimum basilicum L, GC-MS
Abstract
Background: The Ocimum basilicum plant, which belongs to the Lamiaceae family, has been used for a very long time in traditional medicine to treat a variety of conditions, including cancer, convulsions, epilepsy, gout, nausea, sore throat, toothaches, bronchitis, rhinitis, mental weariness, cold, spasms, and as a first aid therapy for snakebites and wasp stings. For medical purposes, the leaves, seeds, and essential oils of the plant are utilized to treat a variety of ailments, including but not limited to: cough, pyrexia, anxiety, gripe, worms, infectious disorders, acne, diarrhea, headaches, constipation, warts, and kidney dysfunction.
Objective: In the current study, the bioactive components of the extract of Ocimum basilicum leaves are investigated, as well as the anti-microbial properties of these fractions in vitro.
Materials and Methods: In order to conduct the GC–mass spectrometry (MS) study of the ethanolic Leaves Extract of Ocimum basilicum L., a Thermo Scientific Trace Ultra GC that was interfaced with a Thermo Fisher Scientific SpA was utilized. Antimicrobial activity of the Ocimum basilicum is being investigated by screening. Phytochemicals and the solvents that were employed for each extract served as the controls for this experiment.
Results: The major constituents were menthone lactone, Diterpenoid SP-II, Pulegone, Sesquiterpene lactone, menthyl acetate, alpha-Cadinol, Linalool, alpha-Bergamotene, beta-Bisabolol, 2,3-dihydroxypropyl elaidate, isopropyl palpitate, 2-methoxy-4-(1-propyl)phenol, α-cubene, vanillin, 1-methyl-3-(1-methyl)benzene, 1,4-diethylbenzene, hexadecanoic acid methyl ester. According to the type of extract (methanol, Ethyl acetate fraction, and Ethanol fraction) recorded 22.41±0.31, 21.39±0.30, and 23.05 ±0.32 respectively in Streptococcus faecalis. While recorded 16.58±0.25, 17.32±0.26, and 22.00±0.30 for Bacillus subtilis . At the same time record 13.09±0.19, 17.00±0.26, and 19.07±0.28 Streptococcus pyogenes. While recorded 15.00±0.25, 21.96±0.30, and 24.65±0.32 Pseudomonas aeruginosa in comparison with Rifambin 21.00±0.30 and Bacteracin 23.41±0.31. The metabolites of Ocimum basilicum exhibited significant activity against Staph. aureus (20.39±0.31).
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References
Dhama, K.; Sharun, K.; Gugjoo, M.B.; Tiwari, R. A comprehensive review on chemical profile and pharmacological activities of Ocimum basilicum. Food Rev. Int. 2023, 39, 119–147.
Da-Silva, F.; Santos, R.H.S.; Diniz, E.R.; Barbosa, L.C.A.; Casali, V.W.D.; De-Lima, R.R. Content and composition of basil essential oil at two different hours in the day and two seasons. Rev. Bras. De Plants Med. 2003, 6, 33–38.
Sajjadi, S. Analysis of the essential oils of two cultivated basil (Ocimum basilicum L.) from Iran. Daru 2006, 14, 128–130.
Hussain, A.I.; Anwar, F.; Sherazi, S.T.H.; Przybylskmi, R. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem. 2008, 108, 986–995.
Benedec, D.; Vlase, L.; Hanganu, D.; Oniga, I. Antioxidant potential and polyphenolic content of Romanian Ocimum basilicum. Dig. J. Nanomater. Biostructures 2012, 7, 1263–1270.
Lee, J.; Scagel, C.F. Chicoric acid found in basil (Ocimum basilicum L.) leaves. Food Chem. 2009, 115, 650–656.
Kwee, E.; Niemeyer, E. Variations in phenolic compositions and antioxidant properties among 15 basil (Ocimum basilicum L.) cultivars. Food Chem. 2011, 128, 1044–1050.
Mandal AK, Poudel M, Neupane NP, Verma A, Mandal AK, Neupane NP, Verma A, Poudel M. Phytochemistry, Pharmacology, and Applications of (Tulsi). In Edible Plants in Health and Diseases; Springer: Singapore, 2022; pp. 135–174.
Chen YH, Wu JX, Yang SF, Yang CK, Chen TH, Hsiao YH. Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells. Cancers. 2022; 14:1824.
Nugroho C, Mirnia E, Cumagun CJR. Antifungal Activities of Sweet Basil (Ocimum basilicum L) Aqueous Extract against Sclerotium rolfsii, Causal Agent of Damping-Off on Tomato Seedling. Agrivita J Agr Sci. 2019;41(1):149-57.
Rumengan IFM, Mandey L, Citraningtiyas G and Luntungan AH. Antihyperglicemic capacity of basil (Ocimum basilicum L.) leaves extracts coated with the marine fish scales derived nanochitosan. IOP Conf Ser: Mater Sci Eng 2019; 567:012023.
Güez, C.; de Souza, R.; Fischer, P. Evaluation of basil extract (Ocimum basilicum L.) on oxidative, anti-genotoxic and antiinflammatory effects in human leukocytes cell cultures exposed to challenging agents. Braz. J. Pharm. Sci. 2017, 53, e15098.
Oliveira JS, Porto LA, Estevam CS, Siqueira RS, Alves PB, Niculau ES, et al. Phytochemical screening and anticonvulsant property of Ocimum basilicum leaf essential oil. Bol. Latinoam. Caribe. Plant Med Aromat. 2009;8:195–202.
Klimankova E, Holadova K, Hajslova J, Cajka T, Poustka J, Koudela M. Aroma profiles of five basil (Ocimum basilicum L.) cultivars grown under conventional and organic conditions. Food Chem. 2008;107:464–72.
Keita SM, Vincent C, Schmit JP, Belanger A. Essential oil composition of Ocimum basilicum L., O. gratissimum L. and O. suave L. in the Republic of Guinea. Flavour Fragr J. 2000;15:339–41.
Vani SR, Cheng SF, Chuah CH. Comparative study of volatile compounds from genus Ocimum. Am J Appl Sci. 2009;6:523–8.
Zheljazkov VD, Cantrel CL, Evans WB, Ebelhar MW, Coker C. Yield and composition of Ocimum basilicum L. and Ocimum sanctum L. grown at four locations. HortScience. 2008;43:737–41.
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