Molecular Diagnosis by Polymerase chain reaction method (PCR) of Aspergillus Fungi Isolated from the Air of the area between the two Holy Mosques in Holy Karbala
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Abstract
The study included molecular diagnosis of some types of Aspergillus sp. fungi for specific areas between the two holy mosques of the city of Karbala. With the aim of identifying the types of Aspergillus present in the region and their importance to community health. Samples were taken from the atmospheric air using an air-sampler system. Using the technique of random polymorphic DNA replication (genetic fingerprinting) after the DNA was extracted from each isolate at a final concentration of pb (524) per 2-3 grams of wet mycelium with 100% purity, using primers ITS1 and ITS4, and by electrophoresis through a gel. Agarose with a concentration of 1.5%, dyed with ethidium bromide, and photographed under ultraviolet light. The genetic fingerprint of two isolates isolated from the air was determined by the appearance of DNA duplication bands for each isolate with one or more primers, and they included: Aspergillus quadrilineatus, Aspergillus terreus, and they were registered in the bank. The global genome with serial numbers OR492487 and OR497735) respectively, with a similarity rate of 100% with the global fungi.
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References
Quorum sensing in fungi–a review. Medical Mycology, 50(4), 337–345.
Baddley, J. W., Pappas, P. G., Smith, A. C., & Moser, S. A. (2003). Epidemiology of Aspergillus terreus at a university hospital. Journal of Clinical Microbiology, 41(12), 5525–5529.
Badran, B., Abd, H. A., & Ramadan, N. a. (2018). Isolation and diagnosis of air fungi inside and outside Tikrit city school buildings. Dirasat Mosiliya, 203–211.
Balina, A., Kebede, A., & Tamiru, Y. (2018). Review on aflatoxin and its impacts on livestock. Journal of Dairy and Veterinary Sciences, 6(2), e555685.
Birren, B., Denning, D., & Nierman, B. (2004). Comparative analysis of an emerging fungal pathogen, Aspergillus terreus. White Paper.
Ellis, M. L., Jimenez, D. R. C., Leandro, L. F., & Munkvold, G. P. (2014). Genotypic and phenotypic characterization of fungi in the Fusarium oxysporum species complex from soybean roots. Phytopathology, 104(12), 1329–1339.
Fisher, M. C., Henk, D. A., Briggs, C. J., Brownstein, J. S., Madoff, L. C., McCraw, S. L., & Gurr, S. J. (2012). Emerging fungal threats to animal, plant and ecosystem health. Nature, 484(7393), 186–194.
Gizachew, D., Szonyi, B., Tegegne, A., Hanson, J., & Grace, D. (2016). Aflatoxin contamination of milk and dairy feeds in the Greater Addis Ababa milk shed, Ethiopia. Food Control, 59, 773–779.
Holland, S. M. (2010). Chronic granulomatous disease. Clinical Reviews in Allergy & Immunology, 38, 3–10.
Kirk, P. M., Cannon, P. F., Minter, D. W., & Stalpers, J. A. (2008). Ainsworth & Bisby’s Dictionary of the fungi. CAB International. Wallingford, United Kingdom.
Mezzari, A., Perin, C., Santos Junior, S. A., & Bernd, L. A. G. (2002). Airborne fungi in the city of Porto Alegre, Rio Grande do Sul, Brazil. Revista Do Instituto de Medicina Tropical de São Paulo, 44, 269–272.
Mishra, V., Nag, V. L., Tandon, R., & Awasthi, S. (2010). Response surface methodology-based optimisation of agarose gel electrophoresis for screening and electropherotyping of rotavirus. Applied Biochemistry and Biotechnology, 160, 2322–2331.
Ostrosky-Zeichner, L., Casadevall, A., Galgiani, J. N., Odds, F. C., & Rex, J. H. (2010). An insight into the antifungal pipeline: selected new molecules and beyond. Nature Reviews Drug Discovery, 9(9), 719–727.
Sambrook, J., & Russell, D. W. (2006). Cycle Sequencing: Dideoxy-mediated Sequencing Reactions Using PCR and End-labeled Primers. Molecular Cloning: A Laboratory Manual, 2, 12–51.