Antifungal Activity and Phytochemical Analysis of Leaves Extract of Medicinal Plant Cassia tora Using GC/MS

Rabab J.H. Al Hasseny (1)
(1) Medical Microbiology, Department of Health Food and Nutrition, College of Food Science, Al-Qasim Green University, Iraq. , Iraq

Abstract

The extensive evidence for the many pharmacological and therapeutic possibilities may be provided by the several activities of Cassia tora. This study used gas chromatography–mass spectrometry to examine the antifungal activity and phytochemical profile of an extract from the medicinal Cassia tora plant.  The resulting crude extracts were further refined with Whatman No. 1 filter paper and kept at 4 °C for future reference. A rotary evaporator operating under vacuum at 40 °C achieved the desired concentration. To determine the chemicals' identities, we used industry-standard techniques to compare their mass spectra to those in the NIST/Wiley internal reference mass spectra library. The antifungal activity of an extract from Cassia tora leaves was tested using the cup plate method, which involves 90% methanol. Dry heat in a hot air oven was used to sterilise petri plates for 1.5 hours at 160 °C.                  Laminitol, Methyl 1-fluoro-4-oxocyclohexane-1-carboxylate, Hexadecanoic acid, alpha-Linolenic acid, 3,7,11,15-Tetramethylhexadec-1-en-3-ol, alpha-Tocopherol acetate, 6-Dodecanone, 5,8-diethyl-7-hydroxy, Methyl beta-D-glucopyranoside, 1,3-Dioleoyl-2-palmitoylglycerol, 2-propylpentyl 14-methylpentadecanoate, 8-Ethylquinoline-3-carboxylic acid, 1-Allyl-4-methoxy-2,3-dimethylbenzene. Methanol, Ethyl acetate, and Ethanol were Candida albicans, (15.00 ± 0.31, 18.55 ± 0.39 and 21.00 ± 0.43 respectively), Candida glabrata (23.00 ± 0.49, 19.08 ± 0.35 and 26.05 ± 0.51 respectively), Trichophyton rubrum (13.00 ± 0.31, 20.74 ± 0.41 and 24.77 ± 0.50 respectively), Microsporum canis (23.09 ± 0.44, 15.95 ± 0.31 and 17.22 ± 0.36 respectively), Aspergillus niger (10.00 ± 0.27, 09.94 ± 0.18 and 18.45 ± 0.37 respectively), Alternaria Alternaria (11.57 ± 0.21, 08.96 ± 0.17 and 15.90 ± 0.36 respectively), and standard antifungal [Voriconazole (VCZ) and Fluconazole (FCZ)] were (29.00 ± 0.54 and 31.79 ± 0.59) respectively.

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References

Kim YM, Lee CH, Kim HG, Lee HS. Anthraquinones isolated from Cassia tora (Leguminosae) seed show an antifungal property against phytopathogenic fungi. Journal of agricultural and food chemistry. 2004;52:6096-100.

Mukherjee PK, Saha K, Saha B, Pal M, Das J. Antifungal activities of the leaf extract of Cassia tora Linn.(Fam. Leguminosae). Phytotherapy Research. 1996;10:521-2.

Hocking P, Pate J. Mobilization of minerals to developing seeds of legumes. Annals of Botany. 1977;41:1259-78.

Garcia-Martinez JL, Sponsel V, Gaskin P. Gibberellins in developing fruits of Pisum sativum cv. Alaska: studies on their role in pod growth and seed development. Planta. 1987;170:130-7.

Lee YP, Puddey IB, Hodgson JM. Protein, fibre and blood pressure: potential benefit of legumes. Clinical and Experimental Pharmacology and Physiology. 2008;35:473-6.

Messina MJ. Legumes and soybeans: overview of their nutritional profiles and health effects. The American journal of clinical nutrition. 1999;70:439s-50s.

de Almeida Costa GE, da Silva Queiroz-Monici K, Reis SMPM, de Oliveira AC. Chemical composition, dietary fibre and resistant starch contents of raw and cooked pea, common bean, chickpea and lentil legumes. Food chemistry. 2006;94:327-30.

Jezierny D, Mosenthin R, Bauer E. The use of grain legumes as a protein source in pig nutrition: A review. Animal Feed Science and Technology. 2010;157:111-28.

Hargrove W. Winter legumes as a nitrogen source for notill grain sorghum. Agronomy Journal. 1986;78:70-4.

Popkin BM, Du S. Dynamics of the nutrition transition toward the animal foods sector in China and its implications: a worried perspective. The Journal of nutrition. 2003;133:3898S- 906S.

Madar Z, Stark AH. New legume sources as therapeutic agents. British Journal of Nutrition. 2002;88:287-92.

Bruneau A, Forest F, Herendeen PS, Klitgaard BB, Lewis GP. Phylogenetic relationships in the Caesalpinioideae (Leguminosae) as inferred from chloroplast trnL intron sequences. Systematic Botany. 2001;26:487-514.

Sharma S, Dangi MS, Wadhwa S, Daniel V, Tiwari A. Antibacterial activity of Cassia tora leaves. Int J Pharmaceutical and Biological Archives. 2010;1:84-6.

Roopashree T, Dang R, Rani RS, Narendra C. Antibacterial activity of antipsoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis. International Journal of Applied research in Natural products. 2008;1:20-8.

Bhatt KD, Gupte HS, Makwana BA, Vyas DJ, Maity D, Jain VK. Calix receptor edifice; scrupulous turn off fluorescent sensor for Fe (III), Co (II) and Cu (II). Journal of fluorescence. 2012;22:1493-500.

Bhatt KD, Makwana BA, Vyas DJ, Mishra DR, Jain VK. Selective recognition by novel calix system: ICT based chemosensor for metal ions. Journal of Luminescence. 2014;146:450-7

Sharma BR, Kumar V, Soni PL. Carboxymethylation of Cassia tora gum. Applied Polymer. 2003;89:3216–3219.

Soni, PL. Pal R. Industrial gum from Cassia tora seeds. Trends in Carbohydrate Chemistry. 1996;2:33–44.

Onalapo JA, Rai PP, Sokomba EN. Preliminary studies on the antimicrobial activities of Cassia tora and Cassia occidentalis medicinal plants. New Vistas of Research. 1993;11:533–536.

Ibrahim M, Khan AA, Tiwari SK, et al. Antimicrobial activity of Sapindus mukorossi and Rheum emodi extracts against Helicobacterium pylori in in vivo studies. World J Gastroenterol. 2006;12(44):7136–7142.

Tsuzuki JK, Svidizinski TIE, Shinobu CS, et al. Antifungal activity of the extracts and saponins from Sapindus saponaria L. Anais da An Acad Bras Cienc. 2007;79(4):577–583.

Jain S, Patil UK. Phytochemical and pharmacological profile of Cassia tora Linn.– An overview. Indian Journal of Natural Products and Resources. 2010;1:430–437

Ibrahim, D. and Osman, H. 1995. Antimicrobial activity of Cassia alata from Malaysia, J. Ethnopharmacol., 45: 151-156.

Jariyalertsak, S., Sirisanthana, T., Supparatpinyo, K. and Nelson, K.E. 1996. Seasonal variation of disseminated Penicilliun marneffei infections in northern Thailand: a clue to the reservoir?, J. Infect. Dis., 173: 1490-1493.

Khan, M.R., Kihara, M. and Omoloso, A.D. 2001. Antimicrobial activity of Cassia alata, Fitoterapia, 72: 561-564.

Manandhar, J.B., Hartman, G.L. and Wang, T.C. 1995. Conidial germination and appressorial formation of Colletotrichum capsici and C. gloeosporioides isolate from pepper, Plant Dis., 79: 361-366.

Palanichamy, S. and Nagarajan, S. 1990. Antifungal activity of Cassia alata leaf extract, J. Ethnopharmacol., 29: 337-340

Authors

Rabab J.H. Al Hasseny
Hasseny, R. J. A. (2024). Antifungal Activity and Phytochemical Analysis of Leaves Extract of Medicinal Plant Cassia tora Using GC/MS. Journal of Current Medical Research and Opinion, 7(11), 3714–3723. https://doi.org/10.52845/CMRO/2024/7-11-3

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