Antifungal Activity and Phytochemical Analysis of Leaves Extract of Medicinal Plant Cassia tora Using GC/MS
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Abstract
The extensive evidence for the many pharmacological and therapeutic possibilities may be provided by the several activities of Cassia tora. This study used gas chromatography–mass spectrometry to examine the antifungal activity and phytochemical profile of an extract from the medicinal Cassia tora plant. The resulting crude extracts were further refined with Whatman No. 1 filter paper and kept at 4 °C for future reference. A rotary evaporator operating under vacuum at 40 °C achieved the desired concentration. To determine the chemicals' identities, we used industry-standard techniques to compare their mass spectra to those in the NIST/Wiley internal reference mass spectra library. The antifungal activity of an extract from Cassia tora leaves was tested using the cup plate method, which involves 90% methanol. Dry heat in a hot air oven was used to sterilise petri plates for 1.5 hours at 160 °C. Laminitol, Methyl 1-fluoro-4-oxocyclohexane-1-carboxylate, Hexadecanoic acid, alpha-Linolenic acid, 3,7,11,15-Tetramethylhexadec-1-en-3-ol, alpha-Tocopherol acetate, 6-Dodecanone, 5,8-diethyl-7-hydroxy, Methyl beta-D-glucopyranoside, 1,3-Dioleoyl-2-palmitoylglycerol, 2-propylpentyl 14-methylpentadecanoate, 8-Ethylquinoline-3-carboxylic acid, 1-Allyl-4-methoxy-2,3-dimethylbenzene. Methanol, Ethyl acetate, and Ethanol were Candida albicans, (15.00 ± 0.31, 18.55 ± 0.39 and 21.00 ± 0.43 respectively), Candida glabrata (23.00 ± 0.49, 19.08 ± 0.35 and 26.05 ± 0.51 respectively), Trichophyton rubrum (13.00 ± 0.31, 20.74 ± 0.41 and 24.77 ± 0.50 respectively), Microsporum canis (23.09 ± 0.44, 15.95 ± 0.31 and 17.22 ± 0.36 respectively), Aspergillus niger (10.00 ± 0.27, 09.94 ± 0.18 and 18.45 ± 0.37 respectively), Alternaria Alternaria (11.57 ± 0.21, 08.96 ± 0.17 and 15.90 ± 0.36 respectively), and standard antifungal [Voriconazole (VCZ) and Fluconazole (FCZ)] were (29.00 ± 0.54 and 31.79 ± 0.59) respectively.
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