Effect of Different Oocytes Collection Techniques on Oocytes Production and Quality of Camel Ovaries
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Camel, Oocyte, Ovarian Anatomy, Follicle Puncture, Follicle Aspiration
Abstract
The purpose of this research was to determine the effect of three distinct approaches on the process of retrieving cumulus oocyte complexes from camel ovaries. On the generation and quality of oocytes from camel ovaries, several techniques were employed, including ovarian dissection, follicle puncture, and follicle aspiration from visible follicles of the ovary. The ovaries were procured from a nearby slaughterhouse in Najaf province and used in the experiments on animals. The oocytes that were collected were roughly categorised into three groups according to the amount of cumulus cells surrounding the mature oocytes and the level of cytoplasmic homogeneity: The oocytes are considered good if they are encased in three or more layers of cumulus cells; they are considered fair if they are encased in two or fewer layers. A lack of cumulus cells means that poor oocytes are nude. The findings of the present study indicated that the mean total number of oocytes collected in each ovary was significantly (P < 0. 05) greater for dissection technique 0. 35 ± 3. 346 as compared with follicle puncture technique 0. 30 ± 2. 461 and aspiration technique 0. 28 ± 2. 115. Altogether, the results achieved in the study underlined a statistically significant rise (P<0.05) in the total number of oocytes, the amount of oocytes per ovary and the percentage of good quality oocytes that is 45. 25 ± 1. 730, and 51. 724%, and 27, 0. 30 ± 1. 038, and 42. 187 percent as compared to follicle aspirating technique at 81, 0. 28 ± 0. 769, and 32. 727%, respectively. Conclusion of this study revealed that ovarian dissection procedure was less time consuming than the follicle puncture and the aspiration techniques in terms of delivering good oocytes in large numbers and with better morphology and quality.
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