Effect of Different Oocytes Collection Techniques on Oocytes Production and Quality of Camel Ovaries

Haider M. Abd Ali (1) , Fawzea G. AL-Gebouri (2) , Hashim M. Al-Rubaei (3) , Razzaq N. Abed (4)
(1) Al-Furat Al-Awsat Technical University, Technical Institute of Al–Musaib, Department of Animal Production Techniques, Iraq. , Iraq
(2) Al-Furat Al-Awsat Technical University., Al-Musaib Technical College, Department of Animal Production Techniques, Iraq , Iraq
(3) Al-Furat Al-Awsat Technical University., Al-Musaib Technical College, Department of Animal Production Techniques, Iraq , Iraq
(4) University of Al-Qasim Green, Iraq. , Iraq

Abstract

The purpose of this research was to determine the effect of three distinct approaches on the process of retrieving cumulus oocyte complexes from camel ovaries. On the generation and quality of oocytes from camel ovaries, several techniques were employed, including ovarian dissection, follicle puncture, and follicle aspiration from visible follicles of the ovary. The ovaries were procured from a nearby slaughterhouse in Najaf province and used in the experiments on animals. The oocytes that were collected were roughly categorised into three groups according to the amount of cumulus cells surrounding the mature oocytes and the level of cytoplasmic homogeneity: The oocytes are considered good if they are encased in three or more layers of cumulus cells; they are considered fair if they are encased in two or fewer layers. A lack of cumulus cells means that poor oocytes are nude. The findings of the present study indicated that the mean total number of oocytes collected in each ovary was significantly (P < 0. 05) greater for dissection technique 0. 35 ± 3. 346 as compared with follicle puncture technique 0. 30 ± 2. 461 and aspiration technique 0. 28 ± 2. 115. Altogether, the results achieved in the study underlined a statistically significant rise (P<0.05) in the total number of oocytes, the amount of oocytes per ovary and the percentage of good quality oocytes that is 45. 25 ± 1. 730, and 51. 724%, and 27, 0. 30 ± 1. 038, and 42. 187 percent as compared to follicle aspirating technique at 81, 0. 28 ± 0. 769, and 32. 727%, respectively. Conclusion of this study revealed that ovarian dissection procedure was less time consuming than the follicle puncture and the aspiration techniques in terms of delivering good oocytes in large numbers and with better morphology and quality.

Full text article

Generated from XML file

References

Abdoon, A.S.S., .2001. Factors affecting follicular population, oocyte yield and quality in camels (Camelus dromedarius) ovary with special reference to maturation time in vitro. Anim Reprod Sci, 66, 71- 79.

AL-Fatlawy M.A. and Hussain, S.O. 2018. Assessment Of Different Techniques for Oocyte Collection for Ivf in the Camel (Camelus dromedarius). Kufa Journal for Veterinary Medical Sciences, 9(1), 76-88.

Alsaadoon, A. A., Atiyah, S. S. and Shanshoul, N. J. 2021. Effect of Follicle Diameter and Culture Medium on The in Vitro Maturation of Sheep Oocytes Using Culture Media Supplemented With Different Concentrations of Sucrose in Local Sheep (Ovis Aries). In IOP Conference Series: Earth and Environmental Science (Vol. 923, No. 1, p. 012038). IOP Publishing.

Atiyah, S.S., Hobi, A.A. and Fakhrildin, M.B.M. .2019. Cryopreservation of Sheep Oocytes and Embryos Using Local, Modifieid Vitrification Tool. iraqi journal of agricultural sciences, 50(Special). https://doi.org/10.36103/ijas.v50iSpecial.192.

Das, G.K., Jain, G.C., Solanki, V.S and Tripathi, V.N .1996. Efficacy of various collection methods for oocyte retrieval in buffalo. Theriogenology, 46, 1403- 1411.

Farah, Z. and Fischer, A., .2004. Milk and Meat from the Camel. Handbook on Products and Processing. vdf Hochschulverlag AG,ETH Zentrum, CH-8092 Zürich.

Farahavar A., Shahne A., Kohram H. and Vahedi V. 2010. Effect of melatonin on in vitro maturation of bovine oocytes. African J. Biotechnol. 9, 2579-2583.

Hammad, M.E., Gabr, Sh.A., El-Ratel, I.T and Gad, M. A .2014. Efficacy of different collection techniques on yield and quality of Egyptian buffalo oocytes. J Animal and Poultry Prod, Mansoura Univ, 5(7), 413- 422

Hoque S.A.M., Kabiraj S.K., Khandoker M.A.M.Y., Mondal A. and Tareq K.M.A . 2011. Effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation and fertilization of goat oocytes. African J. Biotechnol. 10, 9177-9181.

Khatir, H. and Anouassi, A .2006. The first dromedary (Camelus dromedarius) offspring obtained from in vitro matured, in vitro fertilized and in vitro cultured abattoir-derived oocytes. Theriogenology, 65, 1727- 1736.

Kouamo, J. and Kharche, SD .2014. Dose dependent effect of pregnant mare serum gonadotropin and human chorionic gonadotropin on in vitro maturation of goat oocyte. Indian J Anim Sci 84: 410-414.

Mantovani R., Rota A., Penzo N. and Bittante G . 1999. Com- parison among oocytes recovery methods for bovine embryo production in vitro. Pp. 235-235 in Proc. 13th A.S.P.A. Cong., Piacenza

Nandi S, Ravindranatha B, Gupta P. and Sarma P. 2002. Timing of sequential changes in cumulus cells and first polar body extrusion during in vitro maturation of buffalo oocytes. Theriogenology.;57:1151-9. https://doi.org/10.1016/S0093-691X(01)00709-9

Rameez, A; Razzaque,W. A. A; Bhat, A. H; Reshi 1. A; Kose, M. and Bhat, F. H . 2017. Efficacy of Different Harvesting Techniques on Oocyte Recovery from Goat Ovaries. International Journal of Livestock ,Research.,7: 180- 185

SAS, .1996. SAS/Stat. User’s Guide Static’s, Ver., 6.06, fourth ed. SAS Institute Inc., Cary, NC.

Skidmore, J.A . 2005. Reproduction in dromedary camels: an update. Anim Reprod, 2(3), 161. 171.

Skidmore, J.A and Billah, M . 2012.The main challenges of artificial insemination and embryo transfer in the dromedary camel. Proceeding of the ICAR Satellite on camelid a Reproduction ,3rd -5 th August ,Vancouver , Canada,pp.51-56.

Wang, Z.G., Xu, Z.R. and Yu, S.D . 2007. Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat. Czech J Anim. Sci. 52, 21-25.

Wani, N.A., Wani, G.M., Khan M.Z. and Salahudin, S . 2000. Effect of oocyte harvesting techniques on in vitro maturation and in vitro fertilization

Authors

Haider M. Abd Ali
Fawzea G. AL-Gebouri
Hashim M. Al-Rubaei
Razzaq N. Abed
Ali, H. M. A., AL-Gebouri , F. G., Al-Rubaei , H. M., & Abed, R. N. (2024). Effect of Different Oocytes Collection Techniques on Oocytes Production and Quality of Camel Ovaries. Journal of Current Medical Research and Opinion, 7(09), 3525–3530. https://doi.org/10.52845/CMRO/2024/7-9-3

Article Details