In vitro Antimicrobial Activity of Plant Extracts on Pseudomonas aeruginosa and Screening of Bioactive Chemical Compounds
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Pseudomonas aeruginosa, Secondary metabolites, Antibacterial
Abstract
Aims and Objectives: This research aimed to analyze the bioactive chemical products of Pseudomonas aeruginosa and evaluate the antibacterial, antifungal and in vitro antimicrobial activities of plant extracts against Pseudomonas aeruginosa.
Method: The chemical components known as bioactives, which are sometimes referred to as secondary metabolites, were examined using gas chromatography-mass spectrometry (GC-MS) techniques. Subsequently, the antibacterial , antifungal activity of the methanolic extract of Pseudomonas aeruginosa was assessed in vitro.
Results: The GC-MS analysis of Pseudomonas aeruginosa detected the presence of the following: The compounds listed include Nonadecanamide, N-[3-[5,8-bis[3-[acetyl(hydroxy)amino]propyl]-3,6,9,12,15,18-hexaoxo-1,4,7,10,13,16-hexazacyclooctadec-2-yl]propyl]-N-hydroxyacetamide, 16-hexazacyclooctadec-2-yl]propyl]-N-hydroxyacetamide, Methyl 2-(2-acetyl-3,5-dihydroxyphenyl)acetate, 1-Cyclohexyloctadecane,, bis(2-propylheptyl) benzene-1,2-dicarboxylate, 2-Ethyl-3,5-dimethylpyridine, decan-4-ylcyclohexane, methyl 1-methylpiperidine-3-carboxylate, beta-Methylacetylcholine, 8-hydroxy-9,11-octadecadiynoic acid, O-Acetyl-N-propionylhydroxylamine, Glycyl-D-asparagine, beta-Hydroxyasparagine, and D-Leucine, methyl ester. The metabolites of Pseudomonas aeruginosa exhibited significant activity against Escherichia coli (15.28±0.05). Pseudomonas aeruginosa metabolites was very highly active against Aspergillus flavus (12.80 ± 0.03). Origanum vulgare (Crude) (20.06±0.18) was very highly active against Pseudomonas aeruginosa.
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