Secondary Metabolite Products from Enterococcus faecalis Using GC/MS and In vitro Evaluation of Its Antimicrobial Activity of Some Medicinal Plant Extracts
Abstract
ABSTRACT
Aims and Objective: The objectives of this study were to analyze Enterococcus faecalis bioactive chemical compounds and assess the antibacterial, antifungal, and in vitro antimicrobial activity of plant extracts on this organism.
Method: Gas chromatography-mass spectrometry (GC-MS) methods were used to investigate bioactives, which are chemical substances frequently referred to as secondary metabolites, before the in vitro antibacterial and antibacterial activity of the Enterococcus faecalis methanolic extract was assessed.
Results: The GC-MS investigation of Enterococcus faecalis demonstrated the presence of the following: 2-Butanol , 8,11-Octadecadiynoic acid , 6-Acetyl-β-d-mannose , Lactose , Cyclopropanebutanoic acid , 9-Hexadecenoic acid , E-11-Hexadecenoic acid , D-Glucose , α-D-Glucopyranoside , O-α-D-glucopyranosyl-(1.fwdarw.3)-β- ,2,3-dihydro-3,5-dihydroxy-6-methyl-, n-Hexadecanoic acid , 9-Octadecenoic acid , Octadecanoic acid , 9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol,(3β,5Z,7E)- 2(5H)-Furanone, D-Glucose, 6-O-α-D-galactopyranosyl, α-D-Glucopyranoside, Bicyclo[2.2.1]heptane-2-carboxylic acid isobutyl-amide, 2H-Oxecin-2-one.3.4.7.8.9.10-hexahydro-4-hydroxy-10-methyl, Maltol, D-Glucose,6-O-α-D-galactopyranosyl, 1-Gala-l-ido-octonic lactone, Acetamide , l-(+)-Ascorbic acid 2,6-dihexadecanoate, D-fructose , diethyl mercaptal , pentaacetate, Octadecanal ,2 –bromo, L-Ascorbic acid , 6-octadecanoate. Enterococcus faecalis metabolites was very highly active against Staphylococcus epidermidis (9.92±0.05). Enterococcus faecalis metabolites was very highly active against Alternaria alternate (10.00 ± 0.07). Cassia angustifolia (Crude) (Crude) (10.48±0.13) was very highly active against Enterococcus faecalis.
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